Planarian EST Database


Dr_sW_001_B05

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_001_B05
         (500 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P32006|PROF1_STRPU  Profilin (SpCoel1)                          33   0.38 
sp|Q58347|PUR2_METJA  Phosphoribosylamine--glycine ligase (G...    32   1.1  
sp|Q01369|GBLP_NEUCR  Guanine nucleotide-binding protein bet...    30   3.2  
sp|Q94981|ARI1_DROME  Ariadne-1 protein (Ari-1)                    30   3.2  
sp|P54032|RS24_METJA  30S ribosomal protein S24e                   29   5.5  
sp|Q12397|STCA_EMENI  Putative sterigmatocystin biosynthesis...    28   9.3  
sp|P31939|PUR9_HUMAN  Bifunctional purine biosynthesis prote...    28   9.3  
sp|Q6GH45|MPRF_STAAR  Probable lysylphosphatidylglycerol syn...    28   9.3  
sp|Q19020|DYHC_CAEEL  Dynein heavy chain, cytosolic (DYHC)         28   9.3  
sp|Q8FDW4|SAT_ECOL6  Serine protease sat precursor (Secreted...    28   9.3  
>sp|P32006|PROF1_STRPU Profilin (SpCoel1)
          Length = 142

 Score = 33.1 bits (74), Expect = 0.38
 Identities = 42/141 (29%), Positives = 63/141 (44%), Gaps = 18/141 (12%)
 Frame = +2

Query: 2   SWDAWGANLDGYM--ASSFTEINNITIASSNGAVCISRSKEGPTPDAN--------EIAA 151
           SWD++  NL      AS  T  +   I   +G+   +     PT D +        E+A 
Sbjct: 2   SWDSYVDNLIAQSKDASGTTHCDKACIIGKDGSAWTTM----PTSDTSNNLKLTPEEMAN 57

Query: 152 LVKYLTSKQ-----GSGLTYGGHKLMFTREIEDSIMIFNIIGASKGALALS-KTASTNFV 313
           + K   SK       SG+   G K  F RE EDS +   ++G  KG  +L+ +++ T  V
Sbjct: 58  IAKCFKSKDFAAFMSSGIYVNGTKYQFLRE-EDSKL---VLGKKKGEGSLTLQSSKTAIV 113

Query: 314 ILACGAGPDKN--SQAVGVNA 370
           I  C  G  +   ++AVGV A
Sbjct: 114 IGHCPEGGQQGNLNKAVGVIA 134
>sp|Q58347|PUR2_METJA Phosphoribosylamine--glycine ligase (GARS) (Glycinamide
           ribonucleotide synthetase) (Phosphoribosylglycinamide
           synthetase)
          Length = 444

 Score = 31.6 bits (70), Expect = 1.1
 Identities = 18/47 (38%), Positives = 25/47 (53%)
 Frame = -3

Query: 153 NAAISFASGVGPSLLLLMHTAPLEEAIVILLISVKLDAI*PSKFAPQ 13
           +A   FA  V P L ++   APL E +V LL  + + A+ P K A Q
Sbjct: 54  DAVKEFAEKVKPDLAVIGPEAPLGEGVVDLLEEMGISAVGPKKLAAQ 100
>sp|Q01369|GBLP_NEUCR Guanine nucleotide-binding protein beta subunit-like protein
           (Cross-pathway control WD-repeat protein cpc-2)
          Length = 316

 Score = 30.0 bits (66), Expect = 3.2
 Identities = 22/73 (30%), Positives = 35/73 (47%), Gaps = 2/73 (2%)
 Frame = +2

Query: 59  INNITIASSNGAVCISRSKEGPTP--DANEIAALVKYLTSKQGSGLTYGGHKLMFTREIE 232
           IN +TI S +G++C S  K+G T   D NE   L     + +   L +  ++        
Sbjct: 195 INAVTI-SPDGSLCASGGKDGTTMLWDLNESKHLYSLNANDEIHALVFSPNRYWLCAATS 253

Query: 233 DSIMIFNIIGASK 271
            SI+IF++   SK
Sbjct: 254 SSIIIFDLEKKSK 266
>sp|Q94981|ARI1_DROME Ariadne-1 protein (Ari-1)
          Length = 503

 Score = 30.0 bits (66), Expect = 3.2
 Identities = 10/18 (55%), Positives = 13/18 (72%)
 Frame = +1

Query: 280 CIEQNC*HQFCYSCLWSW 333
           C  QNC ++FC+ CL SW
Sbjct: 309 CKNQNCKNEFCWVCLGSW 326
>sp|P54032|RS24_METJA 30S ribosomal protein S24e
          Length = 101

 Score = 29.3 bits (64), Expect = 5.5
 Identities = 17/39 (43%), Positives = 24/39 (61%)
 Frame = -3

Query: 336 GPAPQARITKLVLAVLLNASAPLLAPIMLKIIIESSISR 220
           GP P  +  KL LA +LNA+  LL  I+ KI+ E+ + R
Sbjct: 27  GPTPTFKDVKLKLAAILNANKDLL--IVEKIVEEAGMQR 63
>sp|Q12397|STCA_EMENI Putative sterigmatocystin biosynthesis polyketide synthase (PKS)
          Length = 2181

 Score = 28.5 bits (62), Expect = 9.3
 Identities = 16/46 (34%), Positives = 22/46 (47%)
 Frame = +2

Query: 59  INNITIASSNGAVCISRSKEGPTPDANEIAALVKYLTSKQGSGLTY 196
           INN + A  N AV +   ++ P P+  EIA         Q S L+Y
Sbjct: 796 INNFSAAGGNTAVLV---EDAPVPELGEIAGSFGEAAGNQSSELSY 838
>sp|P31939|PUR9_HUMAN Bifunctional purine biosynthesis protein PURH [Includes:
           Phosphoribosylaminoimidazolecarboxamide
           formyltransferase (AICAR transformylase); IMP
           cyclohydrolase (Inosinicase) (IMP synthetase) (ATIC)]
          Length = 592

 Score = 28.5 bits (62), Expect = 9.3
 Identities = 32/123 (26%), Positives = 55/123 (44%), Gaps = 9/123 (7%)
 Frame = +2

Query: 29  DGYMASSFTEINNITIASS--NGAVCISRSKEGPTPDANEIAALV-KYLTSKQGSGLTYG 199
           DG +A  + E   +TI S   NG  C+ +  +   PD NE+  L   +L+ K+ +G+   
Sbjct: 339 DGIIAPGYEE-EALTILSKKKNGNYCVLQMDQSYKPDENEVRTLFGLHLSQKRNNGVV-- 395

Query: 200 GHKLMFT------REIEDSIMIFNIIGASKGALALSKTASTNFVILACGAGPDKNSQAVG 361
             K +F+      +++ +S +   I+     A    K   +N V  A      KN Q +G
Sbjct: 396 -DKSLFSNVVTKNKDLPESALRDLIV-----ATIAVKYTQSNSVCYA------KNGQVIG 443

Query: 362 VNA 370
           + A
Sbjct: 444 IGA 446
>sp|Q6GH45|MPRF_STAAR Probable lysylphosphatidylglycerol synthetase (LPG synthetase)
           (Multiple peptide resistance factor)
          Length = 840

 Score = 28.5 bits (62), Expect = 9.3
 Identities = 25/99 (25%), Positives = 45/99 (45%), Gaps = 2/99 (2%)
 Frame = +2

Query: 125 TPDANEIAALVKYLTSKQGSGLTYGGHKLMFTREIEDSIMIFNIIGASKGALALSKTAST 304
           T D  ++   +  +     +GL+     ++F   + D+ +I N I   +  L        
Sbjct: 120 THDKKKLVHFISLILISMLTGLSLLSLLIVF--HVFDASLILNKITWVRWVLYAVSLFLP 177

Query: 305 NFVILACGAGPDKNSQAVGV--NAVKCVEFLNKSL*LAF 415
            F+I +    PDKN++ VG+    V CVE+L  ++ L F
Sbjct: 178 LFIIYSMVRPPDKNNRYVGLYCTLVSCVEWLAAAVVLYF 216
>sp|Q19020|DYHC_CAEEL Dynein heavy chain, cytosolic (DYHC)
          Length = 4568

 Score = 28.5 bits (62), Expect = 9.3
 Identities = 13/26 (50%), Positives = 17/26 (65%)
 Frame = -3

Query: 468 RIKINIEK*YNPNELFSINANYNDLF 391
           R+K  +E   N NE+FSI + YN LF
Sbjct: 549 RLKSQLESSRNSNEMFSIFSRYNALF 574
>sp|Q8FDW4|SAT_ECOL6 Serine protease sat precursor (Secreted autotransporter toxin sat)
          Length = 1295

 Score = 28.5 bits (62), Expect = 9.3
 Identities = 24/82 (29%), Positives = 37/82 (45%), Gaps = 2/82 (2%)
 Frame = +2

Query: 62  NNITIASSNGAVCISRSKEGPTPDANEIAALVKYLTSKQGSGLTYGGHKLMFTR--EIED 235
           NNI +AS NG V +++         N+  A + +  +K+G  L   GH   FTR    +D
Sbjct: 442 NNIYLASGNGKVILNKDNS----LGNDQYAGIFF--TKRGGTLDLNGHNQTFTRIAATDD 495

Query: 236 SIMIFNIIGASKGALALSKTAS 301
              I N     +  LA++   S
Sbjct: 496 GTTITNSDTTKEAVLAINNEDS 517
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 53,946,632
Number of Sequences: 369166
Number of extensions: 977032
Number of successful extensions: 2638
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2586
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2636
length of database: 68,354,980
effective HSP length: 102
effective length of database: 49,512,010
effective search space used: 3168768640
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)