Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_001_A24
(390 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8JZX4|SPF45_MOUSE Splicing factor 45 (45 kDa splicing f... 37 0.018
sp|Q96I25|SPF45_HUMAN Splicing factor 45 (45 kDa splicing f... 37 0.018
sp|P30539|GLGB_BUTFI 1,4-alpha-glucan branching enzyme (Gly... 32 0.58
sp|Q93Y94|RPO1_NICSY DNA-directed RNA polymerase 1, mitocho... 30 2.2
sp|Q59832|GLGB2_STRCO 1,4-alpha-glucan branching enzyme 2 (... 29 2.9
sp|Q04913|NR4A2_XENLA Orphan nuclear receptor NR4A2 (Orphan... 29 3.8
sp|P0A9T8|YBBA_ECOLI Hypothetical ABC transporter ATP-bindi... 29 3.8
sp|P25586|YCF9_YEAST Hypothetical 37.2 kDa protein in CHA1-... 29 3.8
sp|Q05913|T2FA_DROME Transcription initiation factor IIF al... 28 4.9
sp|P42690|SRK4_SPOLA Tyrosine-protein kinase isoform SRK4 28 6.4
>sp|Q8JZX4|SPF45_MOUSE Splicing factor 45 (45 kDa splicing factor) (RNA-binding motif
protein 17)
Length = 405
Score = 36.6 bits (83), Expect = 0.018
Identities = 35/124 (28%), Positives = 58/124 (46%), Gaps = 4/124 (3%)
Frame = +3
Query: 24 MALYDFDDVGKPKTFSWSGGLTPSIYLMHSHVH-KLQRNAKRQVQHPIPTSKLPPVFDLK 200
M+LYD D+G + S + G + + L+ S + K + + Q ++ L PV DLK
Sbjct: 1 MSLYD--DLGVETSDSKTEGWSKNFKLLQSQLQVKKAALTQAKSQRTKQSTVLAPVIDLK 58
Query: 201 KTIGEGEYIFNLLTGKIEKFNPGSHQLQGLGD---LSYDDPLLLLCVKDEYNPLMPNDYE 371
+ + +I P H GL D + +L+ + DEY+P+ PNDYE
Sbjct: 59 RGGSSDDR-------QIADTPP--HVAAGLKDPVPSGFSAGEVLIPLADEYDPMFPNDYE 109
Query: 372 QISK 383
++ K
Sbjct: 110 KVVK 113
>sp|Q96I25|SPF45_HUMAN Splicing factor 45 (45 kDa splicing factor) (RNA-binding motif
protein 17)
Length = 401
Score = 36.6 bits (83), Expect = 0.018
Identities = 33/124 (26%), Positives = 57/124 (45%), Gaps = 4/124 (3%)
Frame = +3
Query: 24 MALYDFDDVGKPKTFSWSGGLTPSIYLMHSHVH-KLQRNAKRQVQHPIPTSKLPPVFDLK 200
M+LYD D+G + S + G + + L+ S + K + + Q ++ L PV DLK
Sbjct: 1 MSLYD--DLGVETSDSKTEGWSKNFKLLQSQLQVKKAALTQAKSQRTKQSTVLAPVIDLK 58
Query: 201 KTIGEGEYIFNLLTGKIEKFNPGSHQLQGLGD---LSYDDPLLLLCVKDEYNPLMPNDYE 371
+ + + + H GL D + +L+ + DEY+P+ PNDYE
Sbjct: 59 RGGSSDDR---------QIVDTPPHVAAGLKDPVPSGFSAGEVLIPLADEYDPMFPNDYE 109
Query: 372 QISK 383
++ K
Sbjct: 110 KVVK 113
>sp|P30539|GLGB_BUTFI 1,4-alpha-glucan branching enzyme (Glycogen branching enzyme) (BE)
(1,4-alpha-D-glucan:1,4-alpha-D-glucan
6-glucosyl-transferase)
Length = 639
Score = 31.6 bits (70), Expect = 0.58
Identities = 17/54 (31%), Positives = 27/54 (50%)
Frame = -1
Query: 186 LGGAWKWEWGVALVFSHSFVICEHVSA*DIWRESNHHSMKMFLVYQHHQNHIKP 25
LG A+KW G + H F CE++ +R+ H+ M + Y +N+I P
Sbjct: 383 LGFAFKWNMG----WMHDF--CEYMKLDPYFRQGAHYMMTFAMSYNDSENYILP 430
>sp|Q93Y94|RPO1_NICSY DNA-directed RNA polymerase 1, mitochondrial precursor (T7
bacteriophage-type single subunit RNA polymerase 1)
(NsRpoT-A)
Length = 1002
Score = 29.6 bits (65), Expect = 2.2
Identities = 16/45 (35%), Positives = 24/45 (53%)
Frame = +3
Query: 99 YLMHSHVHKLQRNAKRQVQHPIPTSKLPPVFDLKKTIGEGEYIFN 233
Y+M +H K QR A ++V P +L PVF T+G ++ N
Sbjct: 454 YIMRTHGAKQQREAVKRV----PKKQLEPVFQALDTLGNTKWRLN 494
>sp|Q59832|GLGB2_STRCO 1,4-alpha-glucan branching enzyme 2 (Glycogen branching enzyme 2)
(BE 2) (1,4-alpha-D-glucan:1,4-alpha-D-glucan
6-glucosyl-transferase 2)
Length = 741
Score = 29.3 bits (64), Expect = 2.9
Identities = 16/54 (29%), Positives = 27/54 (50%)
Frame = -1
Query: 186 LGGAWKWEWGVALVFSHSFVICEHVSA*DIWRESNHHSMKMFLVYQHHQNHIKP 25
LG KW G + H + E+V+ + R +HH M +VY + +N++ P
Sbjct: 491 LGFGLKWNMG----WMHDSL--EYVAHEPVHRRYHHHEMTFSMVYAYSENYVLP 538
>sp|Q04913|NR4A2_XENLA Orphan nuclear receptor NR4A2 (Orphan nuclear receptor NURR1)
(Nerve growth factor-induced protein I-B homolog)
Length = 577
Score = 28.9 bits (63), Expect = 3.8
Identities = 23/94 (24%), Positives = 44/94 (46%), Gaps = 3/94 (3%)
Frame = +3
Query: 54 KPKTFSWSGGLTPSIYLMHSHVHKLQRNAKRQVQHPIPTSKLPPVFDL---KKTIGEGEY 224
KPK + S + L+ +H+ + ++K + SK L K++ + +
Sbjct: 331 KPKQIAESSPVDLINSLVRAHIDSIPSSSK------LDYSKFQETVPLQLEKESSVDVQQ 384
Query: 225 IFNLLTGKIEKFNPGSHQLQGLGDLSYDDPLLLL 326
++LL+G +E + ++QG DL +D LLL
Sbjct: 385 FYDLLSGSLEVIRKWAEKIQGFVDLPKEDQDLLL 418
>sp|P0A9T8|YBBA_ECOLI Hypothetical ABC transporter ATP-binding protein ybbA
sp|P0A9U0|YBBA_SHIFL Hypothetical ABC transporter ATP-binding protein ybbA
sp|P0A9T9|YBBA_ECO57 Hypothetical ABC transporter ATP-binding protein ybbA
Length = 228
Score = 28.9 bits (63), Expect = 3.8
Identities = 11/28 (39%), Positives = 19/28 (67%)
Frame = +3
Query: 162 IPTSKLPPVFDLKKTIGEGEYIFNLLTG 245
+P + V LKK++G+GE+ ++LTG
Sbjct: 1 MPAENIVEVHHLKKSVGQGEHELSILTG 28
>sp|P25586|YCF9_YEAST Hypothetical 37.2 kDa protein in CHA1-PRD1 intergenic region
Length = 316
Score = 28.9 bits (63), Expect = 3.8
Identities = 15/44 (34%), Positives = 25/44 (56%)
Frame = +3
Query: 132 RNAKRQVQHPIPTSKLPPVFDLKKTIGEGEYIFNLLTGKIEKFN 263
RN +++V P P ++LP DL+ I GEY + +++K N
Sbjct: 241 RNVEKKVYTPFPPAQLPRKVDLE--IESGEYFLSKREKQMKKLN 282
>sp|Q05913|T2FA_DROME Transcription initiation factor IIF alpha subunit (TFIIF-alpha)
(Transcription factor 5, large chain) (TF5A)
Length = 577
Score = 28.5 bits (62), Expect = 4.9
Identities = 21/73 (28%), Positives = 34/73 (46%)
Frame = +3
Query: 69 SWSGGLTPSIYLMHSHVHKLQRNAKRQVQHPIPTSKLPPVFDLKKTIGEGEYIFNLLTGK 248
S+SGG + V +R KR+ P+ ++L F KKT + + +T
Sbjct: 504 SFSGGKVEDYGITEEAV---RRYLKRK---PLTATELLTKFKNKKTPVSSDRLVETMTKI 557
Query: 249 IEKFNPGSHQLQG 287
++K NP H +QG
Sbjct: 558 LKKINPVKHTIQG 570
>sp|P42690|SRK4_SPOLA Tyrosine-protein kinase isoform SRK4
Length = 506
Score = 28.1 bits (61), Expect = 6.4
Identities = 16/54 (29%), Positives = 27/54 (50%), Gaps = 4/54 (7%)
Frame = +3
Query: 120 HKLQRNAKRQVQHPIPTSK----LPPVFDLKKTIGEGEYIFNLLTGKIEKFNPG 269
H+L ++ K +++ P P K +PP D+K I G+Y ++ T F G
Sbjct: 25 HELSQSVKGKIKQPEPKPKPPPQVPPAQDVKYPIYVGKYDYDSRTDDDLSFKKG 78
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 52,687,228
Number of Sequences: 369166
Number of extensions: 1195288
Number of successful extensions: 3109
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3053
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3107
length of database: 68,354,980
effective HSP length: 96
effective length of database: 50,620,420
effective search space used: 1670473860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)