Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_001_A23
(725 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O97902|DDEF1_BOVIN 130-kDa phosphatidylinositol 4,5-biph... 39 0.019
sp|P16884|NFH_RAT Neurofilament triplet H protein (200 kDa ... 36 0.092
sp|O70546|UTX_MOUSE Ubiquitously transcribed X chromosome t... 36 0.092
sp|Q60554|NKX61_MESAU Homeobox protein Nkx-6.1 35 0.16
sp|Q99MA9|NKX61_MOUSE Homeobox protein Nkx-6.1 35 0.16
sp|O07650|TONB_CAMCO Protein tonB 35 0.20
sp|P46582|YLB8_CAEEL Hypothetical protein C34E10.8 in chrom... 35 0.27
sp|Q9UDY2|ZO2_HUMAN Tight junction protein ZO-2 (Zonula occ... 35 0.27
sp|O35762|NKX61_RAT Homeobox protein Nkx-6.1 34 0.35
sp|P40523|YIF5_YEAST Hypothetical 70.7 kDa protein in SNP1-... 34 0.35
>sp|O97902|DDEF1_BOVIN 130-kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1
GTPase-activating protein (PIP2-dependent ARF1 GAP)
(ADP-ribosylation factor-directed GTPase-activating
protein 1) (ARF GTPase-activating protein 1) (Development
and differentiation-enhancing factor 1)
(Differentiation-enhancing factor 1) (DEF-1)
Length = 1129
Score = 38.5 bits (88), Expect = 0.019
Identities = 27/119 (22%), Positives = 46/119 (38%)
Frame = +1
Query: 169 LPSIKKPAANDLASQLRAKQETMKHTSTNSNADNISRESPTPPVQSTQSISSKPPKIDFL 348
LP P S + TST+S I+ P PP +T+ P +
Sbjct: 756 LPGFSTPRDKQRLSYGAFTNQIFVSTSTDSPTSPIAEAPPLPPRNATKGPPGPPSTLPLS 815
Query: 349 SEIKLKTQNRQDGINPPKKIEQPRTPAIPTVNLPFKNPNQSSKPLPSNQDPTTASFVTS 525
++ + +PP RT + P LP PN+ + P ++ P+++S T+
Sbjct: 816 TQTSSGSSTLSKKRSPPPPPGHKRTLSDPPSPLPHGPPNKGAVPWGNDVGPSSSSKTTN 874
>sp|P16884|NFH_RAT Neurofilament triplet H protein (200 kDa neurofilament protein)
(Neurofilament heavy polypeptide) (NF-H)
Length = 831
Score = 36.2 bits (82), Expect = 0.092
Identities = 25/115 (21%), Positives = 47/115 (40%), Gaps = 5/115 (4%)
Frame = +1
Query: 178 IKKPAANDLASQLRAKQETMKHTSTNSNADNISRESPTPPVQSTQSISSKPPKIDF-LSE 354
+K P A A + + ++ + +SP T+ ++ K ++ + E
Sbjct: 584 VKSPEAKPPAKEEAKRPADIRSPEQVKSPAKEEAKSPEKEETRTEKVAPKKEEVKSPVEE 643
Query: 355 IKLKTQNRQDGINPPKKIEQPRTPAIPTVNLPFKN----PNQSSKPLPSNQDPTT 507
+K K PPKK+E+ +TPA P + P ++ KP ++P T
Sbjct: 644 VKAK--------EPPKKVEEEKTPATPKTEVKESKKDEAPKEAQKPKAEEKEPLT 690
>sp|O70546|UTX_MOUSE Ubiquitously transcribed X chromosome tetratricopeptide repeat
protein (Ubiquitously transcribed TPR protein on the X
chromosome)
Length = 1333
Score = 36.2 bits (82), Expect = 0.092
Identities = 24/81 (29%), Positives = 44/81 (54%), Gaps = 1/81 (1%)
Frame = +1
Query: 169 LPSIKKPAANDLASQLRAKQETMKHTSTNSNADNISRESPTPPV-QSTQSISSKPPKIDF 345
LPSI++ + + ++L ++Q M +T+ + +DN S + PPV Q T S P K+
Sbjct: 350 LPSIEEAWSLPIPAELTSRQGAM-NTAQQNTSDNWSGGNAPPPVEQQTHSWCLTPQKLQH 408
Query: 346 LSEIKLKTQNRQDGINPPKKI 408
L +++ N +NP +K+
Sbjct: 409 LEQLRANRNN----LNPAQKL 425
>sp|Q60554|NKX61_MESAU Homeobox protein Nkx-6.1
Length = 364
Score = 35.4 bits (80), Expect = 0.16
Identities = 28/99 (28%), Positives = 42/99 (42%), Gaps = 4/99 (4%)
Frame = +1
Query: 244 TSTNSNADNISRESPTPPVQSTQSISSKPPKIDFLSEIKLKTQN----RQDGINPPKKIE 411
T S++ + S SP+PP+ + KPP LS + Q GIN +
Sbjct: 45 TGPPSSSSSSSSSSPSPPLGAHNPGGLKPPAAGGLSSLSSPPQQLSAATPHGIN--DILS 102
Query: 412 QPRTPAIPTVNLPFKNPNQSSKPLPSNQDPTTASFVTSA 528
+P P LP +P+ SS S+ T+AS +A
Sbjct: 103 RPSMPVASGAALPSASPSGSSSSSSSSASATSASAAAAA 141
>sp|Q99MA9|NKX61_MOUSE Homeobox protein Nkx-6.1
Length = 365
Score = 35.4 bits (80), Expect = 0.16
Identities = 28/96 (29%), Positives = 42/96 (43%), Gaps = 4/96 (4%)
Frame = +1
Query: 253 NSNADNISRESPTPPVQSTQSISSKPPKIDFLSEIKLKTQN----RQDGINPPKKIEQPR 420
+S++ + S SP+PP+ S KPP LS + Q GIN + +P
Sbjct: 49 SSSSSSSSSSSPSPPLGSHNPGGLKPPAAGGLSSLGSPPQQLSAATPHGIN--DILSRPS 106
Query: 421 TPAIPTVNLPFKNPNQSSKPLPSNQDPTTASFVTSA 528
P LP +P+ SS S+ T+AS +A
Sbjct: 107 MPVASGAALPSASPSGSSSSSSSSASATSASAAAAA 142
>sp|O07650|TONB_CAMCO Protein tonB
Length = 232
Score = 35.0 bits (79), Expect = 0.20
Identities = 31/104 (29%), Positives = 46/104 (44%), Gaps = 4/104 (3%)
Frame = +1
Query: 229 ETMKHTSTNSN----ADNISRESPTPPVQSTQSISSKPPKIDFLSEIKLKTQNRQDGINP 396
ET+K N N S PT P Q+ Q S+ PK + + EIK ++ I
Sbjct: 40 ETIKENKFNMAIKHFVQNSSDMKPTQPTQTIQEPSNVQPK-EPVQEIKKIKPRKEKLIAK 98
Query: 397 PKKIEQPRTPAIPTVNLPFKNPNQSSKPLPSNQDPTTASFVTSA 528
PKKI P ++ P K+ N +P+ Q P +S+ + A
Sbjct: 99 PKKIIPPAN--AKAISQPKKDTNM-QQPIMQQQTPQASSYQSVA 139
>sp|P46582|YLB8_CAEEL Hypothetical protein C34E10.8 in chromosome III
Length = 1024
Score = 34.7 bits (78), Expect = 0.27
Identities = 29/113 (25%), Positives = 50/113 (44%)
Frame = +1
Query: 187 PAANDLASQLRAKQETMKHTSTNSNADNISRESPTPPVQSTQSISSKPPKIDFLSEIKLK 366
PAA + S R ET+ ST+S A I T S +++S +PP S++
Sbjct: 844 PAAMTITSNKRKIDETLASESTSSEATLI---HDTTSSSSAETVSGEPP-AKKSSDVSAP 899
Query: 367 TQNRQDGINPPKKIEQPRTPAIPTVNLPFKNPNQSSKPLPSNQDPTTASFVTS 525
+ + +KI++P+TP K+ + + P PS + P A+ +
Sbjct: 900 VPSPE---KEKEKIDRPKTP---------KSSTKRTTPTPSGRTPRAAAIAAN 940
>sp|Q9UDY2|ZO2_HUMAN Tight junction protein ZO-2 (Zonula occludens 2 protein) (Zona
occludens 2 protein) (Tight junction protein 2)
Length = 1190
Score = 34.7 bits (78), Expect = 0.27
Identities = 28/109 (25%), Positives = 45/109 (41%), Gaps = 6/109 (5%)
Frame = +1
Query: 178 IKKPAANDLASQLRAKQETMKHTSTNSNADNISRESPTPPVQSTQSISS------KPPKI 339
+ +PA L S + E ++H ++I + SP P Q ++ SS PP
Sbjct: 937 LDEPAEEPLVSSITRSSEPVQHE------ESIRKPSPEPRAQMRRAASSDQLRDNSPPPA 990
Query: 340 DFLSEIKLKTQNRQDGINPPKKIEQPRTPAIPTVNLPFKNPNQSSKPLP 486
K KTQN+++ + K E P+ N + P S+K P
Sbjct: 991 FKPEPPKAKTQNKEESYDFSKSYEYKSNPSAVAGN---ETPGASTKGYP 1036
>sp|O35762|NKX61_RAT Homeobox protein Nkx-6.1
Length = 365
Score = 34.3 bits (77), Expect = 0.35
Identities = 27/96 (28%), Positives = 42/96 (43%), Gaps = 4/96 (4%)
Frame = +1
Query: 253 NSNADNISRESPTPPVQSTQSISSKPPKIDFLSEIKLKTQN----RQDGINPPKKIEQPR 420
+S++ + S SP+PP+ + KPP LS + Q GIN + +P
Sbjct: 49 SSSSSSSSSSSPSPPLGAHNPGGLKPPAAGGLSSLGSPPQQLSAATPHGIN--DILSRPS 106
Query: 421 TPAIPTVNLPFKNPNQSSKPLPSNQDPTTASFVTSA 528
P LP +P+ SS S+ T+AS +A
Sbjct: 107 MPVASGAALPSASPSGSSSSSSSSASATSASAAAAA 142
>sp|P40523|YIF5_YEAST Hypothetical 70.7 kDa protein in SNP1-GPP1 intergenic region
Length = 627
Score = 34.3 bits (77), Expect = 0.35
Identities = 24/98 (24%), Positives = 41/98 (41%), Gaps = 2/98 (2%)
Frame = +1
Query: 166 DLPSIKKPAANDLASQLRAKQETMKHTSTNSNADNISRESPTPPVQSTQSISSKPPK--I 339
D PS++ P +D + +R+ + K + + A ++S +P PP ST PP
Sbjct: 137 DSPSLRYPEISDSNNDVRSAPDETKRSISPRYASHVSSVTPQPPSAST------PPSQFF 190
Query: 340 DFLSEIKLKTQNRQDGINPPKKIEQPRTPAIPTVNLPF 453
F E+ L+ + P + P P + PF
Sbjct: 191 SFSPEVSLRKNENITPLPTPVPVPVGVPPLAPPPHGPF 228
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 67,933,509
Number of Sequences: 369166
Number of extensions: 1178933
Number of successful extensions: 4037
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3751
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4006
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6510836890
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)