Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_001_A19
(548 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P00354|G3P1_HUMAN Glyceraldehyde-3-phosphate dehydrogena... 147 2e-35
sp|P20287|G3P_SCHMA Glyceraldehyde-3-phosphate dehydrogenas... 144 1e-34
sp|P51469|G3P_XENLA Glyceraldehyde-3-phosphate dehydrogenas... 144 1e-34
sp|P26519|G3PC_PETCR Glyceraldehyde-3-phosphate dehydrogena... 144 2e-34
sp|O57479|G3P_COLLI Glyceraldehyde-3-phosphate dehydrogenas... 140 2e-33
sp|P04406|G3P2_HUMAN Glyceraldehyde-3-phosphate dehydrogena... 140 3e-33
sp|Q9N2D5|G3P_FELCA Glyceraldehyde-3-phosphate dehydrogenas... 140 3e-33
sp|Q05025|G3P_COTJA Glyceraldehyde-3-phosphate dehydrogenas... 139 3e-33
sp|Q39769|G3PC_GINBI Glyceraldehyde-3-phosphate dehydrogena... 139 3e-33
sp|P26988|G3P_PHYIN Glyceraldehyde-3-phosphate dehydrogenas... 139 5e-33
>sp|P00354|G3P1_HUMAN Glyceraldehyde-3-phosphate dehydrogenase, muscle (GAPDH)
Length = 334
Score = 147 bits (370), Expect = 2e-35
Identities = 76/133 (57%), Positives = 93/133 (69%)
Frame = +1
Query: 40 QKYYSGCTGVAKAVGKVIPEI*MAK*LEWLXXXXXXXXXXXDLTCKLKKAAKYDEIKAAI 219
Q TG AKAVGKVIPE+ K DLTC+L+K AKYD+IK +
Sbjct: 203 QNLIPASTGAAKAVGKVIPEL-DGKLTGMAFRVPTANVSVLDLTCRLEKPAKYDDIKKVV 261
Query: 220 KHASETTMKGILAYTEEEVVSQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYSC 399
K ASE +KGIL YTE+EVVS DF G ++SSIFDA AGI+L DTFVKLV+WYDNE+GYS
Sbjct: 262 KEASEGPLKGILGYTEDEVVSDDFNGSNHSSIFDAGAGIELNDTFVKLVSWYDNEFGYSE 321
Query: 400 RVIDLIQHMSTKD 438
RV+DL+ HM++K+
Sbjct: 322 RVVDLMAHMASKE 334
>sp|P20287|G3P_SCHMA Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Major larval
surface antigen) (P-37)
Length = 338
Score = 144 bits (364), Expect = 1e-34
Identities = 76/135 (56%), Positives = 89/135 (65%)
Frame = +1
Query: 37 LQKYYSGCTGVAKAVGKVIPEI*MAK*LEWLXXXXXXXXXXXDLTCKLKKAAKYDEIKAA 216
+Q TG AKAVGKVIP + K DLTC+L K A Y+EIKAA
Sbjct: 204 MQNIIPASTGAAKAVGKVIPAL-NGKLTGMAFRVPTPDVSVVDLTCRLGKGASYEEIKAA 262
Query: 217 IKHASETTMKGILAYTEEEVVSQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYS 396
+K A+ +KGIL YTE+EVVS DF G +SSIFDAKAGI L + FVKLV+WYDNE+GYS
Sbjct: 263 VKAAASGPLKGILEYTEDEVVSSDFVGSTSSSIFDAKAGISLNNNFVKLVSWYDNEFGYS 322
Query: 397 CRVIDLIQHMSTKDH 441
CRV+DLI HM DH
Sbjct: 323 CRVVDLITHMHKVDH 337
Score = 28.9 bits (63), Expect = 8.6
Identities = 14/19 (73%), Positives = 16/19 (84%)
Frame = +2
Query: 2 SSKLWRDGRRCSFRNIIPA 58
SSKLWRDGR + +NIIPA
Sbjct: 193 SSKLWRDGRG-AMQNIIPA 210
>sp|P51469|G3P_XENLA Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Length = 333
Score = 144 bits (363), Expect = 1e-34
Identities = 77/133 (57%), Positives = 92/133 (69%)
Frame = +1
Query: 40 QKYYSGCTGVAKAVGKVIPEI*MAK*LEWLXXXXXXXXXXXDLTCKLKKAAKYDEIKAAI 219
Q TG AKAVGKVIPE+ K DLTC+L+K AKYD+IKAAI
Sbjct: 202 QNIIPASTGAAKAVGKVIPEL-NGKITGMAFRVPTPNVSVVDLTCRLQKPAKYDDIKAAI 260
Query: 220 KHASETTMKGILAYTEEEVVSQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYSC 399
K ASE MKGIL YT+++VVS DF GD +SSIFDA AGI L + FVKLV+WYDNE GYS
Sbjct: 261 KTASEGPMKGILGYTQDQVVSTDFNGDTHSSIFDADAGIALNENFVKLVSWYDNECGYSN 320
Query: 400 RVIDLIQHMSTKD 438
RV+DL+ HM++K+
Sbjct: 321 RVVDLVCHMASKE 333
>sp|P26519|G3PC_PETCR Glyceraldehyde-3-phosphate dehydrogenase, cytosolic
Length = 336
Score = 144 bits (362), Expect = 2e-34
Identities = 76/124 (61%), Positives = 90/124 (72%)
Frame = +1
Query: 61 TGVAKAVGKVIPEI*MAK*LEWLXXXXXXXXXXXDLTCKLKKAAKYDEIKAAIKHASETT 240
TG AKAVGKV+P + K DLT +L+KAA YDEIKAAIKH SET+
Sbjct: 212 TGAAKAVGKVLPAL-NGKLTGMAFRVPTVDVSVVDLTARLEKAATYDEIKAAIKHESETS 270
Query: 241 MKGILAYTEEEVVSQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYSCRVIDLIQ 420
+KGIL YTE++VVS DF GD SSIFDAKAGI L FVK+V+WYDNE+GYS RVIDLI+
Sbjct: 271 LKGILGYTEDDVVSTDFVGDSRSSIFDAKAGIALNGNFVKVVSWYDNEWGYSNRVIDLIR 330
Query: 421 HMST 432
HM++
Sbjct: 331 HMAS 334
>sp|O57479|G3P_COLLI Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Length = 333
Score = 140 bits (354), Expect = 2e-33
Identities = 74/133 (55%), Positives = 91/133 (68%)
Frame = +1
Query: 40 QKYYSGCTGVAKAVGKVIPEI*MAK*LEWLXXXXXXXXXXXDLTCKLKKAAKYDEIKAAI 219
Q TG AKAVGKVIPE+ K DLTC+L+K AKYD+IK +
Sbjct: 202 QNIIPASTGAAKAVGKVIPEL-NGKLTGMAFRVPTPNVSVVDLTCRLEKPAKYDDIKRVV 260
Query: 220 KHASETTMKGILAYTEEEVVSQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYSC 399
K A++ +KGILAYTE++VVS DF GD +SS FDA AGI L D FVKLV+WYDNEYGYS
Sbjct: 261 KAAADGPLKGILAYTEDQVVSCDFNGDSHSSTFDAGAGIALNDHFVKLVSWYDNEYGYSN 320
Query: 400 RVIDLIQHMSTKD 438
RV+DL+ HM++K+
Sbjct: 321 RVVDLMVHMASKE 333
>sp|P04406|G3P2_HUMAN Glyceraldehyde-3-phosphate dehydrogenase, liver (GAPDH)
Length = 335
Score = 140 bits (352), Expect = 3e-33
Identities = 73/134 (54%), Positives = 89/134 (66%)
Frame = +1
Query: 37 LQKYYSGCTGVAKAVGKVIPEI*MAK*LEWLXXXXXXXXXXXDLTCKLKKAAKYDEIKAA 216
LQ TG AKAVGKVIPE+ K DLTC+L+K AKYD+IK
Sbjct: 203 LQNIIPASTGAAKAVGKVIPEL-NGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKV 261
Query: 217 IKHASETTMKGILAYTEEEVVSQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYS 396
+K ASE +KGIL YTE +VVS DF D +SS FDA AGI L D FVKL++WYDNE+GYS
Sbjct: 262 VKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYS 321
Query: 397 CRVIDLIQHMSTKD 438
RV+DL+ HM++K+
Sbjct: 322 NRVVDLMAHMASKE 335
>sp|Q9N2D5|G3P_FELCA Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Length = 333
Score = 140 bits (352), Expect = 3e-33
Identities = 73/133 (54%), Positives = 90/133 (67%)
Frame = +1
Query: 40 QKYYSGCTGVAKAVGKVIPEI*MAK*LEWLXXXXXXXXXXXDLTCKLKKAAKYDEIKAAI 219
Q TG AKAVGKVIPE+ K DLTC+L+KAAKYD+IK +
Sbjct: 202 QNIIPASTGAAKAVGKVIPEL-NGKLTGMAFRVPTPNVSVVDLTCRLEKAAKYDDIKKVV 260
Query: 220 KHASETTMKGILAYTEEEVVSQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYSC 399
K ASE +KGIL YTE++VVS DF D +SS FDA AGI L D FVKL++WYDNE+GYS
Sbjct: 261 KQASEGPLKGILGYTEDQVVSCDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSN 320
Query: 400 RVIDLIQHMSTKD 438
RV+DL+ HM++K+
Sbjct: 321 RVVDLMAHMASKE 333
>sp|Q05025|G3P_COTJA Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Length = 333
Score = 139 bits (351), Expect = 3e-33
Identities = 73/133 (54%), Positives = 90/133 (67%)
Frame = +1
Query: 40 QKYYSGCTGVAKAVGKVIPEI*MAK*LEWLXXXXXXXXXXXDLTCKLKKAAKYDEIKAAI 219
Q TG AKAVGKVIPE+ K DLTC+L+K AKYD+IK +
Sbjct: 202 QNIIPASTGAAKAVGKVIPEL-NGKLTGMAFRVPTPNVSVVDLTCRLEKPAKYDDIKRVV 260
Query: 220 KHASETTMKGILAYTEEEVVSQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYSC 399
K A+E +KGIL YTE++VVS DF GD +SS FDA AGI L D FVKLV+WYDNE+GYS
Sbjct: 261 KAAAEGPLKGILGYTEDQVVSCDFNGDSHSSTFDAGAGIALNDNFVKLVSWYDNEFGYSN 320
Query: 400 RVIDLIQHMSTKD 438
RV+DL+ HM++K+
Sbjct: 321 RVVDLMVHMASKE 333
>sp|Q39769|G3PC_GINBI Glyceraldehyde-3-phosphate dehydrogenase, cytosolic
Length = 340
Score = 139 bits (351), Expect = 3e-33
Identities = 75/124 (60%), Positives = 88/124 (70%)
Frame = +1
Query: 61 TGVAKAVGKVIPEI*MAK*LEWLXXXXXXXXXXXDLTCKLKKAAKYDEIKAAIKHASETT 240
TG AKAVGKV+P + K DLT +L+K A YDEIKAAIK SE
Sbjct: 216 TGAAKAVGKVLPAL-NGKLTGMAFRVPTPDVSVVDLTVRLEKPASYDEIKAAIKEESEGK 274
Query: 241 MKGILAYTEEEVVSQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYSCRVIDLIQ 420
+KGIL YTE++VVS DF GD+ SSIFDAKAGI L+D FVKLV+WYDNE+GYS RVIDLI
Sbjct: 275 LKGILGYTEDDVVSTDFIGDNRSSIFDAKAGIALSDNFVKLVSWYDNEWGYSSRVIDLIV 334
Query: 421 HMST 432
HM++
Sbjct: 335 HMAS 338
>sp|P26988|G3P_PHYIN Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Length = 332
Score = 139 bits (350), Expect = 5e-33
Identities = 76/122 (62%), Positives = 85/122 (69%)
Frame = +1
Query: 61 TGVAKAVGKVIPEI*MAK*LEWLXXXXXXXXXXXDLTCKLKKAAKYDEIKAAIKHASETT 240
TG AKAVGKVIP + K DLT +L A YDEIKAAIK ASE
Sbjct: 208 TGAAKAVGKVIPSL-NGKLTGMSFRVPTADVSVVDLTARLVNPASYDEIKAAIKSASENE 266
Query: 241 MKGILAYTEEEVVSQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYSCRVIDLIQ 420
MKGIL YTE+ VVS DF GD +SSIFDA+AGI LTD FVKLV+WYDNE+GYS RV+DLI+
Sbjct: 267 MKGILGYTEKAVVSSDFIGDSHSSIFDAEAGIALTDDFVKLVSWYDNEWGYSSRVLDLIE 326
Query: 421 HM 426
HM
Sbjct: 327 HM 328
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 57,800,820
Number of Sequences: 369166
Number of extensions: 1030383
Number of successful extensions: 2994
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2455
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2535
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 3833118120
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)